The Effects of Avobenzone on the Swim Velocity and Survival Rate of Danio rerio Larvae
DOI:
https://doi.org/10.29173/eureka28830Keywords:
Avobenzone, Swim Velocity, Survival Rate, Danio rerioAbstract
Background Avobenzone is a UV-A filtering compound found in many personal care products such as sunscreens, cosmetics and soaps. Previous studies found that avobenzone exposure is associated with increased acetylcholinesterase (AChE) activity, which correlates with altered swimming behaviour in aquatic organisms. This study assessed the effects of varying avobenzone concentrations on survival rate and swim velocity in larval zebrafish (Danio rerio).
Methods Survival rate was continuously monitored over a 7 day exposure while swim velocity was recorded with behaviour tracking software at 7 days post fertilization.
Results We found that exposure to avobenzone elicited non-monotonic changes to larval zebrafish swim velocity, while leaving survival rate statistically unaffected.
Conclusions We speculate that avobenzone does not impart high lethality, and that swim velocity changes were caused by energy diversion from locomotor activity to toxicant elimination by active transport at high concentrations.
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Abreu, M., Genario, R., Giacomini, A., Demin, K., Lakstygal, A., Amstislavskaya, T., Fontana, B., Parker, M., Kalueff, A. (2020). Zebrafish as a Model of Neurodevelopmental Disorders. Neuroscience. 445: 3-11. https://doi.org/10.1016/j.neuroscience.2019.08.034
Afonso, S., Horita, K., Sousa S., Almeida, I., Amaral, M., Lobão, P., Costa, P., Miranda, M., Esteves da Silva, J., Sousa, LJ. (2014). Photodegradation of avobenzone: Stabilization effect of antioxidants. Journal of Photochemistry and Photobiology B: Biology. 140: 36-40. https://doi.org/10.1016/j.jphotobiol.2014.07.004
Bordalo, D., Cuccaro, A., De Marchi, L., Soares, A., Meucci, V., Battaglia, F., Pretti, C., Freitas, R. (2022). In vitro spermiotoxicity and in vivo adults’ biochemical pattern after exposure of the Mediterranean mussel to the sunscreen avobenzone. Environmental Pollution. 312(119987). https://doi.org/10.1016/j.envpol.2022.119987
Brandão, F., Rodrigues, S., Castro, B., Gonçalves, F., Antunes, S., Nunes, B. (2013). Short-term effects of neuroactive pharmaceutical drugs on a fish species: Biochemical and behavioural effects. Aquatic Toxicology. 144: 218–229. https://doi.org/10.1016/j.aquatox.2013.10.005
Brewer, S., Little, E., DeLo, A., Beauvais, S., Jones, S., Ellersieck, MR. (2001). Behavioral dysfunctions correlate to altered physiology in rainbow trout (Oncorynchus mykiss ) exposed to cholinesterase-inhibiting chemicals. Archives of Environmental Contamination and Toxicology. 40(1): 70–76. https://doi.org/10.1007/s002440010149
Duis, K., Junker, T., Coors, A. (2022). Review of the environmental fate and effects of two UV filter substances used in cosmetic products. Science of the Total Environment. 808(151931) https://doi.org/10.1016/j.scitotenv.2021.151931
Giokas, D., Salvador, A., Chisvert, A. (2007). UV filters: From sunscreens to the human body and the environment. Trends in Analytical Chemistry: TRAC. 26(5): 360–374. https://doi.org/10.1016/j.trac.2007.02.012
Gupta P (2019). Disposition and Fate of Toxicants. Concepts and Applications in Veterinary Toxicology (pp. 27–44).
Lecomte, N., Ehrich, D., Ims, R., Yoccoz, N. (2009). Toward understanding the effect of top predators on ecosystems. F1000 Biology Reports. https://doi.org/10.3410/b1-26
Liu, Y., Wang, Y., Li, N., Jiang, S. (2022). Avobenzone and nanoplastics affect the development of zebrafish nervous system and retinal system and inhibit their locomotor behavior. Science of the Total Environment, 806(150681). https://doi.org/10.1016/j.scitotenv.2021.150681
Łopaciuk A. (2013). Global beauty industry trends in the 21st century. https://toknowpress.net/ISBN/978-961-6914-02-4/papers/ML13-365.pdf
Ma, B., Lu, G., Liu, J., Yan, Z., Yang, H., Pan, T. (2017). Bioconcentration and multi-biomarkers of organic UV filters (BM-DBM and OD-PABA) in crucian carp. Ecotoxicology and Environmental Safety. 141: 178–187. https://doi.org/10.1016/j.ecoenv.2017.03.034
McKight, P., & Najab, J. (2010). Kruskal‐Wallis Test. The Corsini Enclyclopidia of Psycology. https://doi.org/10.1002/9780470479216.corpsy0491
Németh, Z., Pirger, Z., Fodor, I., Óvári, M., Komáromy, A. (2023). Analytical methods for investigating the presence, photoisomerisation-, and degradation kinetics of the UV-A filter avobenzone under aqueous conditions to ensure a more realistic environmental measurement. Journal of Photochemistry and Photobiology A: Chem. 439(114621). https://doi.org/10.1016/j.jphotochem.2023.114621
PubChem. (n.d.). Avobenzone. [Accessed 2024 March 22] https://pubchem.ncbi.nlm.nih.gov/compound/Avobenzone
Sabatos-DeVito, M., Murias, M., Dawson, G., Howell, T., Yuan, A., Marsan, S., Bernier, R., Brandt, C., Chawarska, K., Dzuira, J., Faja, S., Jeste, S., Naples, A., Nelson, C., Shic, F., Sugar, C., Webb, S., McPartland, J. (2019). Methodological considerations in the use of Noldus EthoVision XT video tracking of children with autism in multi-site studies. Biological Psychology. 146(107712). DOI: 10.1016/j.biopsycho.2019.05.012.
Sam, C., & Bordoni, B. (2023). Physiology, Acetylcholine. https://www.ncbi.nlm.nih.gov/books/NBK557825/.
Scott, G., & Sloman, K. (2004). The effects of environmental pollutants on complex fish behaviour: integrating behavioural and physiological indicators of toxicity. Aquatic Toxicology. 68(4): 369–392. https://doi.org/10.1016/j.aquatox.2004.03.016
Shi Y. (2002). Mechanisms of Caspase Activation and Inhibition during Apoptosis. Molecular Cell, 9(3); 459–470. https://doi.org/10.1016/s1097-2765(02)00482-3
Sigma-Aldrich. (n.d.). Avobenzone. [Accessed 2024 March 22] https://www.sigmaaldrich.com/CA/en/product/sial/phr1073?utm_source=bing&utm_medium=cpc&utm_campaign=applied_dsa_pdp_NA_%28bing+ebizpfs%29&utm_id=420338408&utm_content=1197368840831303&msclkid=d22eb65d179f112041e6d14acbd982a8&utm_term=sigmaaldrich
SigmaPlot Version 15.0 (2023). Systat Software, Inc., San Jose, California.
Tierney K. (2011). Behavioural assessments of neurotoxic effects and neurodegeneration in zebrafish. Biochimica Biophysica Acta Molecular Basis for Disease. 1812(3): 381–389. https://doi.org/10.1016/j.bbadis.2010.10.011
Trang, A., Khandhar, P. (2023). Physiology, Acetylcholinesterase. https://www.ncbi.nlm.nih.gov/books/NBK539735/.
Trebše, P., Polyakova, O., Baranova, M., Kralj, M., Dolenc, D., Sarakha, M., Kutin, A., Lebedev, A. (2016). Transformation of avobenzone in conditions of aquatic chlorination and UV-irradiation. Water Research. 101: 95–102. https://doi.org/10.1016/j.watres.2016.05.067
Van der Eerden L. (1982). Toxicity of ammonia to plants. Agriculture and Environment. 7(3–4): 223–235. https://doi.org/10.1016/0304-1131(82)90015-7
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