Enhanced Prokaryotic Expression of Dengue Virus Envelope Protein

Authors

  • Advaita Ganguly University of Alberta
  • Ravindra B. Malabadi University of Alberta
  • Dipankar Das University of Alberta
  • Mavanur R. Suresh University of Alberta
  • Hoon H Sunwoo University of Alberta

DOI:

https://doi.org/10.18433/J3PC80

Abstract

Purpose. To highlight the expression and purification of the recombinant dengue virus type-1 antigen exploiting the codon optimized full length envelope for increased yield in E. coli. Methods. A 6x His tag was inserted at the C terminus to facilitate purification. The purified protein was recognized in Western blot by Monoclonal antibody specific for the tag. The in vitro refolded recombinant protein was used to immunize mice for the development of hybridomas and also analyzed for its biological functionality with heparan sulfate binding assay. Results. The polyclonal anti-sera from the immunized mice were found to recognize the envelope protein thereby establishing the immunogenicity of the protein. Conclusion. The purified envelope protein could potentially be used towards dengue diagnostics and vaccine development efforts.

 

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Author Biography

Hoon H Sunwoo, University of Alberta

Faculty of Pharmacy and Pharmaceutical Sciences

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Published

2013-10-20

How to Cite

Ganguly, A., Malabadi, R. B., Das, D., Suresh, M. R., & Sunwoo, H. H. (2013). Enhanced Prokaryotic Expression of Dengue Virus Envelope Protein. Journal of Pharmacy & Pharmaceutical Sciences, 16(4), 609–621. https://doi.org/10.18433/J3PC80

Issue

Section

Pharmaceutical Sciences; Review Articles