Intracellular and Plasma Trough Concentration and Pharmacogenetics of Telaprevir

Authors

  • Jessica Cusato Department of Medical Sciences, Infectious Diseases Unit, Amedeo di Savoia Hospital, University of Turin, Turin, Italy.
  • Sarah Allegra Department of Medical Sciences, Infectious Diseases Unit, Amedeo di Savoia Hospital, University of Turin, Turin, Italy.
  • Amedeo De Nicolò Department of Medical Sciences, Infectious Diseases Unit, Amedeo di Savoia Hospital, University of Turin, Turin, Italy.
  • Lucio Boglione Department of Medical Sciences, Infectious Diseases Unit, Amedeo di Savoia Hospital, University of Turin, Turin, Italy.
  • Giovanna Fatiguso Department of Medical Sciences, Infectious Diseases Unit, Amedeo di Savoia Hospital, University of Turin, Turin, Italy.
  • Adnan Mohamed Abdi Department of Medical Sciences, Infectious Diseases Unit, Amedeo di Savoia Hospital, University of Turin, Turin, Italy.
  • Giuseppe Cariti Department of Medical Sciences, Infectious Diseases Unit, Amedeo di Savoia Hospital, University of Turin, Turin, Italy.
  • Giovanni Di Perri Department of Medical Sciences, Infectious Diseases Unit, Amedeo di Savoia Hospital, University of Turin, Turin, Italy.
  • Antonio D’Avolio Department of Medical Sciences, Infectious Diseases Unit, Amedeo di Savoia Hospital, University of Turin, Turin, Italy.

DOI:

https://doi.org/10.18433/J3DK6T

Abstract

PURPOSE: Triple therapy for HCV-1 infection consists in boceprevir or telaprevir, ribavirin and PEG-interferon. Telaprevir is a P-glycoprotein substrate and it is metabolized by CYP3A4/5. No data have been published on intracellular penetration of telaprevir. We determined peripheral blood mononuclear cells (PBMCs) and trough plasma S and R telaprevir isomers concentrations; moreover, we evaluated the influence of some single nucleotide polymorphisms (SNPs) on these pharmacokinetic data after 1 month of triple therapy in humans. METHODS: Plasma and intracellular telaprevir concentrations were determined at the end of dosing interval (Ctrough) using ULPC-MS/MS validated methods; allelic discrimination was performed through real-time PCR. RESULTS: Median telaprevir Ctrough plasma concentrations were 2579 ng/mL and 2233 ng/mL for the pharmacologically more active S, and R, enantiomers, respectively, with median S/R plasma ratio of 1.11. In PBMC, the medians were 6863 ng/mL and 1096 ng/mL for S and R, respectively, with median S/R being 5.73. The PBMC:plasma ratio for S was 2.59 for R. Plasma ribavirin concentrations were directly correlated with plasma S-telaprevir concentrations. In linear regression analysis, only CYP24A1_rs2585428 SNP (p=0.003) and body mass index (p=0.038) were able to predict S-telaprevir PBMC concentrations. CONCLUSIONS: Our preliminary data could increase the understanding of mechanisms underlying telaprevir intracellular and plasma exposure, suggesting the implementation of pharmacogenetics in these drug kinetic studies.

 

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Published

2015-05-14

How to Cite

Cusato, J., Allegra, S., De Nicolò, A., Boglione, L., Fatiguso, G., Mohamed Abdi, A., … D’Avolio, A. (2015). Intracellular and Plasma Trough Concentration and Pharmacogenetics of Telaprevir. Journal of Pharmacy & Pharmaceutical Sciences, 18(2), 171–176. https://doi.org/10.18433/J3DK6T

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