in Vitro Effect of Methamphetamine on Proliferation, Differentiation and Apoptosis of Adipose Tissue Stem Cells

In vitro Effect of Methamphetamine

Authors

  • Leyla Anari Department of Biology, Islamic Azad University, Arsanjan Branch, Arsanjan, Iran
  • Davood Mehrabani Stem Cell Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
  • Mahboobeh Nasiri Department of Biology, Islamic Azad University, Arsanjan Branch, Arsanjan, Iran
  • Shahrokh Zare Stem Cell Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
  • Iman Jamhiri Stem Cell Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
  • Jason Acker 5Department of Laboratory Medicine and Pathology, University of Alberta and 6Innovation and Portfolio Management, Canadian Blood Services, Edmonton, AB, Canada

DOI:

https://doi.org/10.18433/jpps31843

Abstract

Purpose: Among abused substances, methamphetamine is a psychostimulant drug widely used recreationally with public health importance. This study investigated the effect of methamphetamine on proliferation, differentiation, and apoptosis of human adipose tissue stem cells (AdSCs). Methods: AdSCs were isolated from human abdominal adipose tissue and were characterized for mesenchymal properties and growth kinetics. MTT assay was undertaken to assess methamphetamine toxicity on proliferation and differentiation properties and apoptosis of hAdSCs. Results: Isolated cells were shown to have mesenchymal properties and a population doubling time (PDT) of 40.1 h. Following methamphetamine treatment, expressions of KI-67 and TPX2 as proliferation genes and Col1A1 and PPARg as differentiation genes decreased. Methamphetamine administration increased the expression of Bax and decreased Bcl-2 genes responsible for apoptosis. Conclusions: Our data suggested when AdSCs were exposed to methamphetamine, it decreased proliferation and differentiation properties of stem cells together with an increase in apoptosis. These findings can be added to the literature, especially when methamphetamine is used recreationally for weight loss purposes.

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Published

2022-01-10

How to Cite

Anari, L., Mehrabani, D., Nasiri, M., Zare, S., Jamhiri, I., & Acker, J. (2022). in Vitro Effect of Methamphetamine on Proliferation, Differentiation and Apoptosis of Adipose Tissue Stem Cells: In vitro Effect of Methamphetamine. Journal of Pharmacy & Pharmaceutical Sciences, 25, 69–76. https://doi.org/10.18433/jpps31843

Issue

Vol. 25 (2022): Pages 1-

Section

Pharmaceutical Sciences; Original Research Articles

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