A Nanoparticle Delivery of Plasmid Encoding Hepatocyte Growth Factor for Gene Therapy of Silicosis in Mice

Authors

  • Haiying Duan 1Institute of Pharmacy & Pharmacology, University of South China, 28 Western Changsheng Road, Hengyang, Hunan, 421000, China Institute of Radiation Medicine, 27 Taiping Road, Beijing 100850, China
  • Peng Gao The second affiliated hospital of Xuzhou medical university, China
  • Xiaochen Cheng Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing 100850, China
  • Yuxin Lu Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing 100850, China
  • Chunsheng Hu National & Local Joint Engineering Research Center of Targeted and Innovative Therapeutics, College of Pharmacy & International Academy of Targeted Therapeutics and Innovation, Chongqing University of Arts and Sciences, Chongqing, 402160, China
  • Xuefeng Zhu
  • Xiaoying Wang
  • Dujuan Li
  • Fengjun Xiao Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing 100850, China
  • Li Du Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing 100850, China
  • Yunmei Liu Institute of Pharmacy & Pharmacology, University of South China, Hengyang, Hunan, China, 421000
  • Qinglin Zhang Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing 100850, China

DOI:

https://doi.org/10.18433/jpps32218

Abstract

Purpose: Silicosis is a serious occupational disease that is characterized by pulmonary infiltrates and fibrosis and is often refractory to current treatments. New therapeutic strategies for silicosis are needed. Hepatocyte growth factor (HGF) is a latent anti-inflammatory and anti-fibrotic growth factor. Methods: We prepared a polyethyleneimine-polyethylene glycol/pHGF/hyaluronic acid (PEG-PEI/pHGF/HA) nanomaterials loaded with plasmid DNA encoding HGF gene to increase its transfection efficiency. The characterization, including DNA entrapment efficiency, morphology, particle size, and zeta-potential of PEG-PEI/pHGF/HA was studied. And a PEG-PEI/pHGF/HA (N/P=30:1) nanoparticle with low toxicity and high transfection efficiency was used in treatment for silicosis in mice. Results: The results showed that the human HGF expression in the lungs of the mice was increased, and the inflammatory cell infiltration and fibrous collagen deposition was significantly reduced. Conclusion: Therefore, PEG-PEI/pHGF/HA nanoparticle warrant further investigation and may be a potential therapeutic strategy for silicosis.

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Published

2021-10-10

How to Cite

Duan, H., Gao, P., Cheng, X., Lu, Y., Hu, C., Zhu, X., … Zhang, Q. (2021). A Nanoparticle Delivery of Plasmid Encoding Hepatocyte Growth Factor for Gene Therapy of Silicosis in Mice . Journal of Pharmacy & Pharmaceutical Sciences, 24, 488–498. https://doi.org/10.18433/jpps32218

Issue

Vol. 24 (2021): Pages: 400-

Section

Pharmaceutical Sciences; Original Research Articles

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