Inhibition of Human Liver Cytochrome P450 by Star Fruit Juice

Authors

  • Jiang-Wei Zhang Laboratory of Pharmaceutical Resource Discovery, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Zhongshan Road, Dalian, China
  • Yong Liu Laboratory of Pharmaceutical Resource Discovery, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Zhongshan Road, Dalian, China
  • Jie Cheng Laboratory of Pharmaceutical Resource Discovery, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Zhongshan Road, Dalian, China
  • Wei Li Laboratory of Pharmaceutical Resource Discovery, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Zhongshan Road, Dalian, China
  • Hong Ma Laboratory of Pharmaceutical Resource Discovery, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Zhongshan Road, Dalian, China
  • Hong-Tao Liu Laboratory of Pharmaceutical Resource Discovery, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Zhongshan Road, Dalian, China
  • Jie Sun The Second Affiliated Hospital of Dalian Medical University, Dalian, China
  • Li-Ming Wang The Second Affiliated Hospital of Dalian Medical University, Dalian, China
  • Yu-Qi He University of Traditional Chinese Medicine, Shanghai, China
  • Yun Wang University of Traditional Chinese Medicine, Shanghai, China
  • Zheng-Tao Wang University of Traditional Chinese Medicine, Shanghai, China
  • Ling Yang Institute of Chemical Physics

DOI:

https://doi.org/10.18433/J30593

Abstract

Purpose. To examine the inhibitory effects of star fruit (Averrhoa carambola) juice towards seven major cytochrome P450 (CYP) isoforms and NADPH-cytochrome P450 reductase (CPR). Methods. The inhibitory effects of star fruit juice (0.5 to 5%, v/v) against the activities of seven CYP isoforms including CYP1A2, CYP2A6, CYP2C8, CYP2C9, CYP2D6, CYP2E1, CYP3A4 and CPR were examined in human liver microsomes. To identify time-dependent inhibition, star fruit juice (2.5%, v/v) was preincubated with microsomes and a NADPH-generating system for 0-15 min, and then the extent of inhibition towards seven CYP isoforms were examined. Results. Star fruit juice (5.0%, v/v) was found to inhibit all the activities of CYP isoforms tested by more than 70%. Based on the half inhibition values (%, v/v), the inhibitory effects towards different CYP isoforms were in the following order: CYP2A6 (0.9) > CYP1A2 (1.4) > CYP2D6 (1.6) > CYP2E1 (2.0) > CYP2C8 (2.2) > CYP2C9 (3.0) > CYP3A4 (3.2). Time-dependent inhibition was not observed towards any of the tested CYP isoforms. In addition, star fruit juice was found not to inhibit the activity of CPR. Conclusions. Star fruit juice inhibited the seven CYP isoforms tested, with the strongest inhibitory effect against CYP2A6 and the least towards CYP3A4.

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Author Biography

Ling Yang, Institute of Chemical Physics

Dr. Ling Yang, Laboratory of Pharmaceutical Resource Discovery, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China, e-mail: yling@dicp.ac.cn

Published

2007-10-12

How to Cite

Zhang, J.-W., Liu, Y., Cheng, J., Li, W., Ma, H., Liu, H.-T., Sun, J., Wang, L.-M., He, Y.-Q., Wang, Y., Wang, Z.-T., & Yang, L. (2007). Inhibition of Human Liver Cytochrome P450 by Star Fruit Juice. Journal of Pharmacy & Pharmaceutical Sciences, 10(4), 496–503. https://doi.org/10.18433/J30593

Issue

Section

Pharmaceutical Sciences; Review Articles