TY - JOUR AU - Kam, Antony AU - Li, Kong M AU - Razmovski-Naumovski, Valentina AU - Nammi, Srinivas AU - Chan, Kelvin AU - Grau, Georges E AU - Li, George Q PY - 2015/09/15 Y2 - 2024/03/28 TI - Curcumin Reduces Tumour Necrosis Factor-Enhanced Annexin V-Positive Microparticle Release in Human Vascular Endothelial Cells JF - Journal of Pharmacy & Pharmaceutical Sciences JA - J Pharm Pharm Sci VL - 18 IS - 4 SE - CLOSED. Special Issue - Chief Guest Editor: Basil D Roufogalis; Co-Guest Editors: Emanuel Strehler & Srinivas Nammi DO - 10.18433/J3ZC8G UR - https://journals.library.ualberta.ca/jpps/index.php/JPPS/article/view/24639 SP - 424-433 AB - <p><strong>PURPOSE:</strong> Circulating microparticles have been highlighted as biomarkers of cardiovascular disease state and progression. The aim of this study was to evaluate the effects of curcumin on microparticle release from endothelial cells undergoing TNF-induced cell activation and apoptosis. <strong>METHODS: </strong>This study evaluated the effects of curcumin on microparticle release, cytotoxicity, apoptosis, cell adhesion molecule expression and monocyte adhesion in EAhy926 human endothelial cells. <strong>RESULTS:</strong> The results showed that the numbers of microparticles were increased by tumour necrosis factor (TNF) or the combination of TNF and cycloheximide (CHX). Curcumin attenuated microparticle release caused by TNF or TNF plus CHX treatments. The pretreatment by curcumin not only negated the accelerated cell death and apoptosis caused by TNF and CHX, but also diminished TNF-induced cell activation, as assessed by reduced surface expression of intercellular adhesion molecule 1, and adhesion of monocytes to endothelial monolayers. <strong>CONCLUSION:</strong> Curcumin reduced microparticle release from endothelial cells undergoing cell activation and apoptosis, which supports its protective role in TNF-associated endothelial dysfunction, and highlights its potential use as a nutraceutical agent for vascular inflammatory diseases.</p><p> </p><p>This article is open to <strong>POST-PUBLICATION REVIEW</strong>. Registered readers (see “For Readers”) may <strong>comment</strong> by clicking on ABSTRACT on the issue’s contents page.</p> ER -