(S)--Gingerol enhances glucose uptake in L6 myotubes by activation of AMPK in response to [Ca<sup>2+</sup>]<sub><i>i</i></sub>
AbstractPURPOSE. The aim of this study was to investigate the mechanism of (S)--gingerol in promoting glucose uptake in L6 skeletal muscle cells. METHODS. The effect of (S)--gingerol on glucose uptake in L6 myotubes was examined using 2-[1,2-3H]-deoxy-D-glucose. Intracellular Ca2+ concentration was measured using Fluo-4. Phosphorylation of AMPKα was determined by Western blotting analysis. RESULTS. (S)--Gingerol time-dependently enhanced glucose uptake in L6 myotubes. (S)--Gingerol elevated intracellular Ca2+ concentration and subsequently induced a dose- and time-dependent enhancement of threonine172 phosphorylated AMPKα in L6 myotubes via modulation by Ca2+/calmodulin-dependent protein kinase kinase. CONCLUSION. The results indicated that (S)--gingerol increased glucose uptake in L6 skeletal muscle cells by activating AMPK. (S)--gingerol, a major component of Zingiber officinale, may have potential for development as an antidiabetic agent. This article is open to POST-PUBLICATION REVIEW. Registered readers (see “For Readers”) may comment by clicking on ABSTRACT on the issue’s contents page.
How to Cite
This is an open access journal with with free of charge submission and non-commercial download. At the time of submission, authors will be asked to transfer the copyright to the accepted article to the Journal of Pharmacy and Pharmaceutical Sciences. The author may purchase the copyright for $500 upon which he/she will have the exclusive copyright to the article. Nevertheless, acceptance of a manuscript for publication in the Journal is with the authors' approval of the terms and conditions of the Creative Commons copyright license Creative Common license (Attribution-ShareAlike) License for non-commercial uses.
CLOCKSS system has permission to collect, preserve, and serve this Archival Unit.