Curcumin Reduces Tumour Necrosis Factor-Enhanced Annexin V-Positive Microparticle Release in Human Vascular Endothelial Cells

Authors

  • Antony Kam Nanyang Technological University http://orcid.org/0000-0003-2155-6887
  • Kong M Li Discipline of Pharmacology, Sydney Medical School, University of Sydney, Australia
  • Valentina Razmovski-Naumovski Faculty of Pharmacy, University of Sydney, Australia;The National Institute of Complementary Medicine (NICM), University of Western Sydney, Australia; School of Science and Health, University of Western Sydney, Australia
  • Srinivas Nammi The National Institute of Complementary Medicine (NICM), University of Western Sydney, Australia; School of Science and Health, University of Western Sydney, Australia
  • Kelvin Chan Faculty of Pharmacy, University of Sydney, Australia;The National Institute of Complementary Medicine (NICM), University of Western Sydney, Australia; School of Science and Health, University of Western Sydney, Australia
  • Georges E Grau Vascular Immunology Unit, Sydney Medical School and Bosch Institute, The University of Sydney, Australia
  • George Q Li Faculty of Pharmacy, University of Sydney, Australia

DOI:

https://doi.org/10.18433/J3ZC8G

Abstract

PURPOSE: Circulating microparticles have been highlighted as biomarkers of cardiovascular disease state and progression. The aim of this study was to evaluate the effects of curcumin on microparticle release from endothelial cells undergoing TNF-induced cell activation and apoptosis. METHODS: This study evaluated the effects of curcumin on microparticle release, cytotoxicity, apoptosis, cell adhesion molecule expression and monocyte adhesion in EAhy926 human endothelial cells. RESULTS: The results showed that the numbers of microparticles were increased by tumour necrosis factor (TNF) or the combination of TNF and cycloheximide (CHX). Curcumin attenuated microparticle release caused by TNF or TNF plus CHX treatments. The pretreatment by curcumin not only negated the accelerated cell death and apoptosis caused by TNF and CHX, but also diminished TNF-induced cell activation, as assessed by reduced surface expression of intercellular adhesion molecule 1, and adhesion of monocytes to endothelial monolayers. CONCLUSION: Curcumin reduced microparticle release from endothelial cells undergoing cell activation and apoptosis, which supports its protective role in TNF-associated endothelial dysfunction, and highlights its potential use as a nutraceutical agent for vascular inflammatory diseases.

 

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Published

2015-09-15

How to Cite

Kam, A., Li, K. M., Razmovski-Naumovski, V., Nammi, S., Chan, K., Grau, G. E., & Li, G. Q. (2015). Curcumin Reduces Tumour Necrosis Factor-Enhanced Annexin V-Positive Microparticle Release in Human Vascular Endothelial Cells. Journal of Pharmacy & Pharmaceutical Sciences, 18(4), 424–433. https://doi.org/10.18433/J3ZC8G

Issue

Vol. 18 No. 4 (2015)

Section

CLOSED. Special Issue - Chief Guest Editor: Basil D Roufogalis; Co-Guest Editors: Emanuel Strehler & Srinivas Nammi

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