Evaluation by microarray of the potential safety of Sarracenia purpurea L. (Sarraceniaceae) a traditional medicine used by the Cree of Eeyou Istchee

Authors

  • Carolina Cieniak Biology Department, University of Ottawa, Ottawa, ON, Canada.
  • Charlotte McDonald Biology Department, University of Ottawa, Ottawa, ON, Canada.
  • John Nash Office of Biotechnology, Genomics and Population Health, Public Health Agency of Canada, Toronto, ON, Canada.
  • Asim Muhammad Department of Chemistry, University of Ottawa, Ottawa, ON, Canada.
  • Alaa Badawi Office of Biotechnology, Genomics and Population Health, Public Health Agency of Canada, Toronto, ON, Canada.
  • Pierre S Haddad Department of Pharmacology, Université de Montréal, Montréal, QC, Canada.
  • Alain Cuerrier Jardin botanique de Montréal, Institut de recherche en biologie végétale, Université de Montréal, Montréal, QC, Canada.
  • Steffany A.L. Bennett Neural Regeneration Laboratory, Ottawa Institute of Systems Biology, Department of Biochemistry Microbiology and Immunology, University of Ottawa, Ottawa, ON, Canada.
  • Brian C Foster Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON, Canada.
  • John T Arnason Biology Department, University of Ottawa, Ottawa, ON, Canada.

DOI:

https://doi.org/10.18433/J3DC74

Abstract

Purpose: The purpose of this study was to assess safety of the traditional antidiabetic extracts of either S. purpurea or its lead active principle, morroniside at the transcriptional level. The overarching objective was to profile and validate transcriptional changes in the cytochrome P450 family of genes, in response to treatment with S. purpurea ethanolic extract or its lead active, morroniside. Methods: Transcriptional activity was profiled using a 19K human cDNA microarray in C2BBe1 cells, clone of Caco-2 intestinal cells, which are a model of first-pass metabolism (1, 2). Cells were treated with S. purpurea extract for 4 and 24 hrs, as well as the pure compound morroniside for 4 hrs, to determine their effects. Results:  No evidence of cytochrome P450 transcriptome regulation or of transcriptional activation of other diabetes relevant mRNA was detected after rigorous quantitative-PCR validation of microarray results. Conclusion: Our data do not support a transcriptional mechanism of action for either S. purpurea extract or its lead active, morroniside.

 

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Author Biography

Brian C Foster, Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON, Canada.

Adjunct Professor

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Published

2015-10-19

How to Cite

Cieniak, C., McDonald, C., Nash, J., Muhammad, A., Badawi, A., Haddad, P. S., … Arnason, J. T. (2015). Evaluation by microarray of the potential safety of Sarracenia purpurea L. (Sarraceniaceae) a traditional medicine used by the Cree of Eeyou Istchee. Journal of Pharmacy & Pharmaceutical Sciences, 18(4), 562–577. https://doi.org/10.18433/J3DC74

Issue

Vol. 18 No. 4 (2015)

Section

CLOSED. Special Issue - Chief Guest Editor: Basil D Roufogalis; Co-Guest Editors: Emanuel Strehler & Srinivas Nammi

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