In Endothelial Cells, the Activation or Stimulation of Soluble Guanylyl Cyclase Induces the Nitric Oxide Production by a Mechanism Dependent of Nitric Oxide Synthase Activation

Authors

  • Ariane Migliato Martinelli Departamento de Ciências Fisiológicas – Universidade Federal de São Carlos, São Carlos, SP, Brasil.
  • Carla Nascimento dos Santos Rodrigues Departamento de Ciências Fisiológicas – Universidade Federal de São Carlos, São Carlos, SP, Brasil.
  • Thiago Francisco de Moraes Departamento de Ciências Fisiológicas – Universidade Federal de São Carlos, São Carlos, SP, Brasil.
  • Gerson Jhonatan Rodrigues Departamento de Ciências Fisiológicas – Universidade Federal de São Carlos, São Carlos, SP, Brasil.

DOI:

https://doi.org/10.18433/jpps29578

Abstract

Purpose. In endothelial cells, investigate if the soluble guanylate cyclase (sGC) activation or stimulation is able to potentiate the relaxation in vessels. Methods. Aortic and coronary rings with and without endothelium were placed in a myograph and cumulative concentration-effect curves for DETA-NO or ataciguat were performed. Nitric oxide (NO) were measured by fluorescence or by selective electrode in human umbilical endothelial cells (HUVECs) in response to some treatments, including ataciguat, 8-Br-cGMP and A23187. Results. The presence of the endothelium potentiated the relaxation induced by DETA-NO in aortic and coronary rings. In addition, in aortic rings the endothelium potentiated the relaxation induced by ataciguat. In the presence of nitric oxide synthase (NOS) inhibitor, the endothelium effect was abolished to DETA-NO or ataciguat, in both vessels. Ataciguat, 8-Br-cGMP and A23187 were able to induce NO production in HUVECs cells. In the presence of NOS inhibitor, the NO production induced by ataciguat and 8-Br-cGMP was abolished. Conclusions. Our results suggest that in aortic and coronary rings the endothelium potentiates the relaxation induced by activation or stimulation of sGC through a mechanism dependent of NOS activation.

 

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Published

2018-03-30

How to Cite

Martinelli, A. M., Rodrigues, C. N. dos S., Moraes, T. F. de, & Rodrigues, G. J. (2018). In Endothelial Cells, the Activation or Stimulation of Soluble Guanylyl Cyclase Induces the Nitric Oxide Production by a Mechanism Dependent of Nitric Oxide Synthase Activation. Journal of Pharmacy & Pharmaceutical Sciences, 21, 38–45. https://doi.org/10.18433/jpps29578

Issue

Vol. 21 (2018): Pages 1-142

Section

Pharmaceutical Sciences; Original Research Articles

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