Phytochemical Comparison of the Water and Ethanol Leaf Extracts of the Cree medicinal plant, Sarracenia purpurea L. (Sarraceniaceae)

Authors

  • Carolina Cieniak Biology Department, University of Ottawa, Ottawa, ON, Canada.
  • Brendan Walshe-Roussel Biology Department, University of Ottawa, Ottawa, ON, Canada.
  • Rui Liu Biology Department, University of Ottawa, Ottawa, ON, Canada.
  • Asim Muhammad Department of Chemistry, University of Ottawa, ON, Canada.
  • Ammar Saleem Biology Department, University of Ottawa, Ottawa, ON, Canada.
  • Pierre S Haddad Department of Pharmacology, Université de Montréal, Montréal, QC, Canada.
  • Alain Cuerrier Jardin botanique de Montréal, Institut de recherche en biologie végétale, Université de Montréal, Montréal, QC, Canada.
  • Brian C Foster Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON, Canada.
  • John T Arnason Biology Department, University of Ottawa, Ottawa, ON, Canada.

DOI:

https://doi.org/10.18433/J35W27

Abstract

Purpose: The Cree of Eeyou Istchee in Northern Quebec identified Sarracenia purpurea L. as an important plant for the treatment of Type 2 diabetes. Traditionally the plant is used as a decoction (boiling water extract) of the leaf, however, in order to study the extract in a laboratory setting, an 80% ethanol extract was used. In this study, the phytochemistry of both extracts of the leaves was compared and quantified. Methods: Two S. purpurea leaf extracts were prepared, one a traditional hot water extract and the other an 80% ethanol extract. Using UPLC-ESI-MS, the extracts were phytochemically compared for 2 triterpenes, betulinic acid and ursolic acid, using one gradient method and for 10 additional substances, including the actives quercetin-3-O-galactoside and morroniside, using a different method. Results: The concentrations of the nine phenolic substances present, as well as an active principle, the iridoid glycoside morroniside, were very similar between the two extracts, with generally slightly higher concentrations of phenolics in the ethanol extract as expected. However, two triterpenes, betulinic acid and ursolic acid, were 107 and 93 times more concentrated, respectively, in the ethanol extract compared to the water extract. Conclusion: The main phytochemical markers and most importantly the antidiabetic active principles, quercetin-3-O-galactoside and morroniside, were present in similar amounts in the two extracts, which predicts similar bioactivity.

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Author Biography

Brian C Foster, Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON, Canada.

Adjunct Professor

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Published

2015-10-12

How to Cite

Cieniak, C., Walshe-Roussel, B., Liu, R., Muhammad, A., Saleem, A., Haddad, P. S., … Arnason, J. T. (2015). Phytochemical Comparison of the Water and Ethanol Leaf Extracts of the Cree medicinal plant, Sarracenia purpurea L. (Sarraceniaceae). Journal of Pharmacy & Pharmaceutical Sciences, 18(4), 484–493. https://doi.org/10.18433/J35W27

Issue

Vol. 18 No. 4 (2015)

Section

CLOSED. Special Issue - Chief Guest Editor: Basil D Roufogalis; Co-Guest Editors: Emanuel Strehler & Srinivas Nammi

License

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